DNA Testing

Chlamydophila, Polyoma & PBFD DNA Testing

Performed at the University of Georgia

Specimen requirements:
Polyomavirus (APV): heparinized whole blood and/or swab (from patient) or environmental monitoring swab or necropsy swab (make sure to cut the surface of tissues)

PBFD: heparinized whole blood (from patient) or environmental monitoring swab or necropsy swab (make sure to cut the surface of the tissues)

Chlamydophila: heparinized whole blood and/or swab (from patient) or environmental monitoring swab or necropsy swab (make sure to cut the surface of tissues)

Shipping:
Cold pak not necessary

Interpretations:
PBFD: A positive result indicates the target viral DNA sequence is present in the sample. A positive blood sample indicates a contaminated environment to which the bird has been exposed to PBFD. If the feathers are abnormal, a positive blood sample suggests the bird is actively infected. If the feathers are normal, it suggests the bird may be clearing the virus or that it is persistently infected. These birds should be retested after 90 days. If a negative blood result is found with a bird with abnormal feathers, a feather biopsy should be submitted.

APV: A positive result indicates the bird has been exposed to the virus thus representing active infection, exposure, or vaccination. Active shedding can be confirmed through a cloacal swab. Testing should be repeated after 90 days. A negative result shows no viral DNA in the blood and thus suggests this bird is a true negative at the time of sample acquisition.

Chlamydophilia: A positive result indicates the presence of the organism. A positive result from a cloacal swab indicates shedding. A negative result shows no Chlamydophiia DNA and suggests that the bird is a true negative at the time of sample acquisition. Note that a negative result does not necessarily exclude infection.